Chemical reactions within DNA duplexes Cyanogen bromide as an effective oligodeoxyribonucleotide coupling agent

Chemistry of Pyrrolizines; Reactions with Cyanogen Bromide and Trifluoroacetic Anhydride

-Interaction of the pyrrolizine 3 with cyanogen bromide in a tetrahydrofuran/water mixture affords addition to the enamine double bond with formation of 5 which can be aromatized to 6 by silica gel. Reaction of 6 with cyanogen bromide in the same solvent mixture yields the indoline 8a which structure is proved in a chemic/d way by conversion of the product into the aldehyde 8(1. The different r...

Structural and kinetic aspects of chemical reactions in DNA duplexes. Information on DNA local structure obtained from chemical ligation data.

Chemical ligation of oligonucleotides in double-stranded helices has been considered in its structural-kinetic aspect. A study was made of (i) two series of DNA duplexes with various arrangements of reacting groups in the ligation junction induced by mispairing or by alteration of furanose structure (the replacement of dT unit with rU, aU, IU, xU, dxT ones) and of (ii) eight synthetic water-sol...

Bromide, the first effective antiepileptic agent.

Parallel multiplex thermodynamic analysis of coaxial base stacking in DNA duplexes by oligodeoxyribonucleotide microchips.

Parallel thermodynamic analysis of the coaxial stacking effect of two bases localized in one strand of DNA duplexes has been performed. Oligonucleotides were immobilized in an array of three-dimensional polyacrylamide gel pads of microchips (MAGIChips'). The stacking effect was studied for all combinations of two bases and assessed by measuring the increase in melting temperature and in the fre...

Cyanogen Bromide Cleavage and Partial Amino Acid

When samples containing homoserine and homoserine lactone Porcine growth hormone has been cleaved with cyanogen were analyzed, the short column and the first part of the long bromide and the resulting fragments separated by gel filtracolumn (until glutamic acid emerged) were run at 50” instead of tion. The amino acid sequences of the amino-terminal the usua1 55”* Under these conditions homoseri...